LOCUS       40924_21384        5224 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGEMe-phiC31int-p(A)90 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Yonemura N, Tamura T, Uchino K, Kobayashi I, Tatematsu K, Iizuka T, 
            Sezutsu H, Muthulakshmi M, Nagaraju J, Kusakabe T.
  TITLE     PhiC31 integrase-mediated cassette exchange in silkworm embryos
  JOURNAL   Mol. Genet. Genomics 287 (9), 731-739 (2012)
  PUBMED    22842670
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Yonemura N, Kobayashi I, Uchino K, Sezutsu H, Tamura T, Kusakabe T.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2012) Contact:Naoyuki Yonemura National Institute 
            of Agrobiological Sciences (NIAS), MAFF; Ohwashi 1-2, Tsukuba 
            Science City 305-8634, Japan
REFERENCE   3  (bases 1 to 5224)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5224)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Genet.
            Genomics"; date: "2012"; volume: "287"; issue: "9"; pages: "731-739"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-APR-2012) Contact:Naoyuki Yonemura National Institute of 
            Agrobiological Sciences (NIAS), MAFF; Ohwashi 1-2, Tsukuba Science 
            City 305-8634, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    59..83
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             143..1957
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"
     protein_bind    complement(1970..1994)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2218..2307
                     /label=synthetic poly(A)90 region
                     /note="synthetic poly(A)90 region"
     regulatory      2322..2331
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2326..2335
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2366..2384)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2391..2407)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2548..3003
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3081..3185
                     /label=AmpR promoter
     CDS             3186..4043
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4217..4805
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5093..5114
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5129..5159
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5167..5183
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5191..5207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atttaggtga cactatagaa tactcaagct agggtaaaac aaagctggat cctcgatcac
       61 aagtttgtac aaaaaagcag gcttcgaagg agatagaacc aattctctaa ggaaatactt
      121 aaccatggtc gactggatcc gggtggacac gtacgcgggt gcttacgacc gtcagtcgcg
      181 cgagcgcgag aattcgagcg cagcaagccc agcgacacag cgtagcgcca acgaagacaa
      241 ggcggccgac cttcagcgcg aagtcgagcg cgacgggggc cggttcaggt tcgtcgggca
      301 tttcagcgaa gcgccgggca cgtcggcgtt cgggacggcg gagcgcccgg agttcgaacg
      361 catcctgaac gaatgccgcg ccgggcggct caacatgatc attgtctatg acgtgtcgcg
      421 cttctcgcgc ctgaaggtca tggacgcgat tccgattgtc tcggaattgc tcgccctggg
      481 cgtgacgatt gtttccactc aggaaggcgt cttccggcag ggaaacgtca tggacctgat
      541 tcacctgatt atgcggctcg acgcgtcgca caaagaatct tcgctgaagt cggcgaagat
      601 tctcgacacg aagaaccttc agcgcgaatt gggcgggtac gtcggcggga aggcgcctta
      661 cggcttcgag cttgtttcgg agacgaagga gatcacgcgc aacggccgaa tggtcaatgt
      721 cgtcatcaac aagcttgcgc actcgaccac tccccttacc ggacccttcg agttcgagcc
      781 cgacgtaatc cggtggtggt ggcgtgagat caagacgcac aaacaccttc ccttcaagcc
      841 gggcagtcaa gccgccattc acccgggcag catcacgggg ctttgtaagc gcatggacgc
      901 tgacgccgtg ccgacccggg gcgagacgat tgggaagaag accgcttcaa gcgcctggga
      961 cccggcaacc gttatgcgaa tccttcggga cccgcgtatt gcgggcttcg ccgctgaggt
     1021 gatctacaag aagaagccgg acggcacgcc gaccacgaag attgagggtt accgcattca
     1081 gcgcgacccg atcacgctcc ggccggtcga gcttgattgc ggaccgatca tcgagcccgc
     1141 tgagtggtat gagcttcagg cgtggttgga cggcaggggg cgcggcaagg ggctttcccg
     1201 ggggcaagcc attctgtccg ccatggacaa gctgtactgc gagtgtggcg ccgtcatgac
     1261 ttcgaagcgc ggggaagaat cgatcaagga ctcttaccgc tgccgtcgcc ggaaggtggt
     1321 cgacccgtcc gcacctgggc agcacgaagg cacgtgcaac gtcagcatgg cggcactcga
     1381 caagttcgtt gcggaacgca tcttcaacaa gatcaggcac gccgaaggcg acgaagagac
     1441 gttggcgctt ctgtgggaag ccgcccgacg cttcggcaag ctcactgagg cgcctgagaa
     1501 gagcggcgaa cgggcgaacc ttgttgcgga gcgcgccgac gccctgaacg cccttgaaga
     1561 gctgtacgaa gaccgcgcgg caggcgcgta cgacggaccc gttggcagga agcacttccg
     1621 gaagcaacag gcagcgctga cgctccggca gcaaggggcg gaagagcggc ttgccgaact
     1681 tgaagccgcc gaagccccga agcttcccct tgaccaatgg ttccccgaag acgccgacgc
     1741 tgacccgacc ggccctaagt cgtggtgggg gcgcgcgtca gtagacgaca agcgcgtgtt
     1801 cgtcgggctc ttcgtagaca agatcgttgt cacgaagtcg actacgggca gggggcaggg
     1861 aacgcccatc gagaagcgcg cttcgatcac gtgggcgaag ccgccgaccg acgacgacga
     1921 agacgacgcc caggacggca cggaagacgt agcggcgtag gatatctaga cccagctttc
     1981 ttgtacaaag tggtgataat taattaagat cgaatctaga ctgactaata agtataattt
     2041 gtttctatta tgtataagtt aagctaatta cttattttat aatacaacat gactgttttt
     2101 aaagtacaaa ataagtttat ttttgtaaaa gagagaatgt ttaaaagttt tgttacttta
     2161 tagaagaaat tttgagtttt tgtttttttt taataaataa ataaacaact agtgattaaa
     2221 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2281 aaaaaaaaaa aaaaaaaaaa aaaaaaaatc gaattcccgc ggccgccatg gcggccggga
     2341 gcatgcgacg tcgggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
     2401 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     2461 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     2521 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     2581 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2641 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2701 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2761 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2821 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2881 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2941 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     3001 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatcaggtg
     3061 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3121 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3181 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3241 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3301 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3361 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3421 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3481 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3541 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3601 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3661 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3721 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3781 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3841 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3901 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3961 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4021 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4081 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4141 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4201 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4261 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4321 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     4381 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4441 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4501 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4561 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4621 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4681 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4741 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4801 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4861 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4921 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4981 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     5041 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5101 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     5161 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     5221 agct
//
